Director Bert I. Gordon created some of the special ant-effects by shooting magnified images of the Panamanian bullet ant.
As a promotional gimmick for the film theaters would display ant farms in their lobbies - though they weren't allowed near the concession counters.
Joan Collins disliked working with the ant props, claiming they bumped into and scratched the actors, including herself.
The name of the the rental car agency owner is painted on the window as "J. Foote" - James E. Foote worked on this movie as transportation captain.
This was the third Bert I. Gordon movie to claim to be based on the works of H. G. Wells. In actuality all films have little-to-no relation to their source material. Empire of the Ants was also the eighth Gordon movie to deal with the subject of giant monsters!